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language
eng
Author
Yoshida, Hiroshi
Description
Culture conditions suitable for production of PDM phosphatase by the fungus, Fusarium moniliforme, were investigated and established as follows. The medium contained 3% (w/v) glucose, 0.1% (w/v) ammonium sulfate and 0.1% (w/v) yeast extract in 50 mM sodium citrate buffer (pH 5.8) and the temperature was 25℃. In an early stage under these conditions, there was production of another unstable phosphatase presumably specific for phosphomonoesters. After the decline of this phosphatase activity at about 40 h of the culture time, PDM phosphatase began to increase and reached a plateau at about 100 h. This time course of production was slightly different from that observed under the same conditions for ribonuclease F1, another phosphoric acid hydrolyzing enzyme secreted by the fungus.
Subject
phosphatase
culture condition
Fusarium moniliforme
Journal Title
Bulletin of Shimane Medical University
Volume
19
Start Page
53
End Page
56
ISSN
03879097
Published Date
1996-12-01
NCID
AN00107602
Publisher
島根医科大学
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
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