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language
eng
Attribute
Original Paper
Author
Yoshida, Hiroshi
Nishioka, Masaaki
Description
Antibody against ribonuclease (RNase) F1 was raised in a rabbit by intramuscular injection of the protein with Freund’s complete adjuvant. Excess amount of the antibody totally inhibited the enzyme activity on RNA substrate. Quantitative precipitin analysis showed the presence of at least 4 or 5 epitopes (antigenic determinants) on the RNase F1 molecule. Cross-reactivity of the antibody with RNases T1, Ms, U2, St and T2 was examined. Although the double diffusion test detected no cross-reactivity with any of these RNases, Western blot analysis with anti RNase F1 antibody revealed weak cross-reactivity with RNases Ms and T1.
Subject
ribonuclease F1
rabbit antibody
ribonuclease T1 family
Journal Title
島根大学医学部紀要
Volume
33
Start Page
49
End Page
52
ISSN
1880084X
Published Date
2010-12-01
NCID
AA12049432
Publisher
島根大学医学部
Publisher Aalternative
Shimane University Faculty of Medicine
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
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