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File
language
eng
Author
Ishikura, Hiroto
Tsumura, Hiroto
Sato, Yukie
Kobayashi, Shotai
Description
It is well established that c-myc expression is down-regulated during induction of differentiation in various leukemia cell lines including HL-60 human myeloid leukemia cells. Previously we have shown that c-myc protein expression was specifically down-regulated in G0/G1 phase of the cell cycle during induction of granulocytic differentiation with dimetylsulfoxide (DMSO) in HL-60 cells. In the present study, we examined whether c-myc protein expression is also down-regulated in G0/G1 phase during monocytic differentiation of HL-60 cells. HL-60 cells can be induced to differentiate along monocytic lineage by treatment with 1,25 dihydroxyvitamin D3 (D3). C-myc protein expression was down-regulated after 72h of treatment with D3 in HL-60 cells. Simultaneous analysis of protein level and cell cycle using a two-color flow cytometry showed that the down-regulation of c-myc protein expression occurred specifically in G0/G1 phase. Down-regulation of c-myc protein expression in G0/G1 phase was more clearly shown by using synchronized cell population that was arrested at G0/G1 phase by serum starvation. Furthermore, G0/G1-synchronized cells showed a higher susceptibility to differentiation in response to D3. These results suggest that c-myc protein is down-regulated in G0/G1-specific phase during not only granulocytic differentiation but also monocytic differentiation, and down-regulation of c-myc protein expression in G0/G1 phase is involved in the program of differentiation of HL-60 cells.
Subject
c-myc
differentiation
cell cycle
HL-60 cells
Journal Title
Shimane journal of medical science
Volume
16
Issue
2
Start Page
45
End Page
49
ISSN
03865959
ISSN(Online)
24332410
Published Date
1998-12-01
NCID
AA00841586
Publisher Aalternative
Shimane Medical University
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
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