(1) After the injections of Zn its amount in needles changed thus ; 2 hours later, no changes were found ; 19 hours later, the amount of Zn increased 4 - 5 times that in the normal condition before the injection, and a month later, increased 6 - 10 times.
(2) The effect of partial cutting of branches on the movement of injected chemicals (Acid Magenta) was as follows ;-
We prepared 2 sample trees, one being a control tree and the other a tree in which all branches of the lower 3/4 parts of the crown were cut out.
In both trees, an upward movement ot Acid Magenta injected into the trunks was seen in the xylem and a downward in the xylem and the phloem.
The upper limit of the movement in the control tree in 1 - 2 days after the injection, was 2 - 3 times as high as that of the treaetd, and the lower limit of the latter was 1.5 times as low as that of the former, measured from the injection point.
It seemed that the cutting of branches lessened the ascending velocity of sap in the xylem.
(3) The absorbability of Acid Magenta into the trunk per hour was at its maximum at noon, and lessened 30-40% in the morning or in the evening.
(4) The Acid Magenta moved upward only along the xylem in the injection side to the level of the lower part of the crown, where, moving on upward, it took its direction into all branches, and at the level of the upper crown it filled the tracheids in all sides of the trunk.