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Title Transcription
マガキゾク シゼン コウザツ コタイ ノ ニタイリツ イデンシ カイセキ
Title Alternative
A biallelic microsatellite DNA analysis for natural hybrid Crassostrea oysters
File
language
jpn
Author
Iwasaki, Takeshi
Tanaka, Tomomi
Iidzuka, Yusuke
Hishida, Yasuhiro
Sheng-Tai
Description
Pacific oyster Crassostrea gigas and Portuguese oyster C. angulata are morphologically
related species that are sympatrically distributed at nearshore blackish areas in
the subtropical zone of East Asia. Although these two species have thus far been known as inter-fertilizable under controlled conditions, molecular-based studies on mitochondrial DNA analysis verified obvious genetic differences between them. Huvet et al. (2004) reported a novel microsatellite DNA marker enabling to discriminate allelic sequence variants for C. gigas and C. angulata. In this study, we developed a rapid, reliable, and secure PCR-RFLP analysis of the biallelic microsatellite marker to diagnose hybrid oysters between C. gigas and C. angulata in wild stocks. An array of wild oyster specimens of C. gigas haplotypes and C. angulata haplotypes collected from Japan, Taiwan, and Korea were subjected to PCR amplification of the microsatellite marker and subsequent RFLP assay using FastDigest® Bsp1407I
restriction enzyme, followed by microchip electrophoretic diagnosis of both PCR and RFLP products. It took about 2 hours for the whole process of our analysis, and the frequencies of biallelic variants observed in C. gigas and C. angulata haplotype stocks were at the equivalent level of those of the previous report. In addition, a high frequency of the allelic variants for C. angulata in C. gigas haplotype stock in Tokyo Bay suggests the occurrence of natural hybrid oysters between C. gigas and C. angulata.
Subject
Crassostrea gigas
Crassostrea angulata
natural hybrid
microsatellite locus
microchip electrophoresis
Journal Title
Laguna : 汽水域研究
Volume
16
Start Page
13
End Page
18
ISSN
21852995
Published Date
2009-06
NCID
AN10439529
Publisher
島根大学汽水域研究センター
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
Gyoseki ID
e22302
OAI-PMH Set
Estuary Research Center