| File | |
| Title |
大腸菌・L-型菌NC7からのカルシウム結合性タンパク質
|
| Title |
Study on Calcium-Binding Protein from Escherichia coli L-form NC7
|
| Title Transcription |
ダイチョウキン Lガタキン NC7 カラノ カルシウム ケツゴウセイ タンパクシツ
|
| Creator |
Yawata Toshio
Onoda Tetsuo
|
| Source Title |
島根大学理学部紀要
Memoirs of the Faculty of Science, Shimane University
|
| Volume | 29 |
| Start Page | 37 |
| End Page | 43 |
| Journal Identifire |
ISSN 03879925
|
| Descriptions |
Abstract
A calmodulin-like protein (CaLP) that has been regarded as a Ca2+-dependent modulator protein in Escherichia coli L-form NC7 was purified by fluphenazine-sepharose 4B affinity chromatography. The molecular weight of the CaLP was approx. 18,000 kDa on SDS-Polyacrylamide gel electrophoresis and isoelectric point of 4.5 was confirmed by electric focusing gel (pH 3 to 10). The CaLP were heat stable and exhibited Ca2+-binding property. Further, the CaLP stimulated bovine hart phosphodiesterase activity. These results suggest the presence of Ca2+-regulatory system in prokaryotes.
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
島根大学理学部
The Faculty of Science, Shimane University
|
| Date of Issued | 1995-12-25 |
| Access Rights | open access |
| Relation |
[NCID]
AN00108106
|
