File
language
eng
Attribute
Original article
Author
Ishikura, Hiroto
Maniwa, Yasuhisa
Munemasa, Shoso
Tsumura, Hiroto
Kobayashi, Shotai
Description
Oncogene products, c-myc and bcl-2 protein, play an important role in the decision of the fate of cells, such as proliferation, differentiation and apoptosis. Terminally differentiated HL-60 human leukemia cells express low levels of both c-myc and bcl-2 protein, and die by apoptosis. We have previously reported that down-regulation of c-myc protein was sufficient for the induction of apoptosis in HL-60 cells, while down-regulation of bcl-2 protein was not the case. To understand regulatory mechanism between c-myc and bcl-2 protein expression, we examined the effects of c-myc or bcl-2 antisense oligonucleotides on the expression levels of bcl-2 or c-myc protein, respectively. Our experiments showed that c-myc antisense down-regulated bcl-2 protein expression, whereas bcl-2 antisense did not change the levels of c-myc protein expression. These results suggest that bcl-2 protein is regulated sequentially at downstream of the decrease in c-myc protein expression during the process of differentiation induction program. Our study identified c-myc protein as a possible primary candidate rather than bcl-2 in the gene targeting therapy of leukemia.
Journal Title
Shimane journal of medical science
Volume
20
Issue
1
Start Page
9
End Page
11
ISSN
03865959
ISSN(Online)
24332410
Published Date
2002-06-01
NCID
AA00841586
Publisher Aalternative
Shimane Medical University
NII Type
Departmental Bulletin Paper
Format
PDF
Text Version
出版社版
OAI-PMH Set
Faculty of Medicine
Remark
http://ci.nii.ac.jp/vol_issue/nels/AA00841586_jp.html