Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p-binding domain

RNA Volume 23 Issue 6 Page 968-981 published_at 2017-06
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Title
Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p-binding domain
Creator
Van Roon Anne-Marie M.
Oubridge Chris
Sposito Benedetta
Newman Andrew J.
Seraphin Bertrand
Nagai Kiyoshi
Source Title
RNA
Volume 23
Issue 6
Start Page 968
End Page 981
Journal Identifire
ISSN 1355-8382
EISSN 1469-9001
Subjects
splicing; U2 snRNP; SF3b; RNA binding; RRM ( Other)
Language
eng
Resource Type journal article
Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
Date of Issued 2017-06
Access Rights metadata only access
Relation
[DOI] 10.1261/rna.059378.116
[PMID] 28348170
Remark We thank Yasushi Kondo for collecting data on the RRM1-Cusl (290-368)p complex crystals and LMB mass spectrometry facility for MALDI-TOF analysis of proteolytic fragments, Dr. Stephen McLaughlin for helping with fluorescence anisotropy data analysis, and the staff at Diamond Light Source beamline 102 and European Synchrotron Radiation Facility beamline ID14-3 for their assistance. We also thank Elena Conti and Sebastian Falk for communicating their unpublished results. We are grateful to Lisa Strittmatter and Pei-chun Lin for critical reading of the manuscript. This project was supported by the Medical Research Council (MC_U105184330), a Wellcome Trust Travelling fellowship, and an MRC career development fellowship (E.O.). B.Sp. was supported by Humanitas Research Foundation. B.Se. was supported by the CNRS, the CERBM-IGBMC and INSERM, the grant ANR-10LABX-0030-INRT, a French State fund managed by the Agence Nationale de la Recherche under the frame program Investissements d'Avenir ANR-10-IDEX-0002-02, and the Ligue Contre le Cancer (Equipe Labellisee 2014).