| ファイル情報(添付) |
Simple and Effective Gap-Repair Cloning Using Short Tracts of Flanking Homology in Fission Yeast
2.46 MB
エンバーゴ :
2021-03-29
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| タイトル |
Simple and Effective Gap-Repair Cloning Using Short Tracts of Flanking Homology in Fission Yeast
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| 著者 |
MATSUO Yuzy
KISHIMOTO Hayafumi
HORIUCHI Tomitaka
TANAE Katsuhiro
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| 収録物名 |
Bioscience, Biotechnology, and Biochemistry
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| 巻 | 74 |
| 号 | 3 |
| 開始ページ | 685 |
| 終了ページ | 689 |
| 収録物識別子 |
ISSN 0916-8451
EISSN 1347-6947
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| 内容記述 |
その他
Gap-repair cloning for plasmid construction in budding yeast is very effective and often used. In contrast, the same method is not widely used in fission yeast, because of a shortage of information on it. Here we describe simple and effective gap-repair cloning for plasmid construction using short tracts of flanking homology. By this method, we combined concentrated DNA fragments with short (20 bp) tracts of flanking homology with the marker gene or the pre-existing gene module. In addition, we found that this method can be applied to one-step cloning of multiple DNA fragments to construct a fusion gene.
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| 主題 | |
| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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| 発行日 | 2010-03-23 |
| 出版タイプ | Accepted Manuscript（出版雑誌の一論文として受付されたもの。内容とレイアウトは出版社の投稿様式に沿ったもの） |
| アクセス権 | オープンアクセス |
| 関連情報 |
[DOI] 10.1271/bbb.90967
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