| File |
Simple and Effective Gap-Repair Cloning Using Short Tracts of Flanking Homology in Fission Yeast
2.46 MB
エンバーゴ :
2021-03-29
|
| Title |
Simple and Effective Gap-Repair Cloning Using Short Tracts of Flanking Homology in Fission Yeast
|
| Creator |
MATSUO Yuzy
KISHIMOTO Hayafumi
HORIUCHI Tomitaka
TANAE Katsuhiro
|
| Source Title |
Bioscience, Biotechnology, and Biochemistry
|
| Volume | 74 |
| Issue | 3 |
| Start Page | 685 |
| End Page | 689 |
| Journal Identifire |
ISSN 0916-8451
EISSN 1347-6947
|
| Descriptions |
Gap-repair cloning for plasmid construction in budding yeast is very effective and often used. In contrast, the same method is not widely used in fission yeast, because of a shortage of information on it. Here we describe simple and effective gap-repair cloning for plasmid construction using short tracts of flanking homology. By this method, we combined concentrated DNA fragments with short (20 bp) tracts of flanking homology with the marker gene or the pre-existing gene module. In addition, we found that this method can be applied to one-step cloning of multiple DNA fragments to construct a fusion gene.
|
| Subjects | |
| Language |
eng
|
| Resource Type | journal article |
| Publisher |
Japan Society for Bioscience, Biotechnology, and Agrochemistry
|
| Date of Issued | 2010-03-23 |
| Publish Type | Accepted Manuscript |
| Access Rights | open access |
| Relation |
[DOI] 10.1271/bbb.90967
|
