チャとツツジのExobasidium菌による肥大組織の電子顕微鏡による観察

その他

Hypertrophied leaves of Thea sinensis L. and Rhododendron indicum Sweet infected by Exobasidium app were studied under electron mycroscope. These leaves were fixed in lper cent OsO_4-- phosphate buffer(pH7.4) and embedded in Epon 812. Sections were made with Hitachi UM-3 ultrotome with glass knives and examinations were carried out with HS-6 electron microscope. The results were shown in Figs.1 ～ 10. In the hypertrophied tissues, lamella structures of chroloplasts did not developed and chroloplasts became to the amyloplast-like structures(Figs. 3, 4). High electr on-dense parts in vacuoles were consisted of fine granulars(Figs. 5, 6, 7) Hyphae were recognized in inter cellular space, middle lamella and cell wall of the suscept cells(Figs. 8, 9, 10) but were not invaginated to the plant cell. Frequently, fine filalmentous hyphal tips were observed in the cell wall of suscept tissues. Starch like material and peculiar membrane structure were recoginzed in the hyphae as shown in Fig. 8.