ダイコン子葉の葉緑体発生に対する4−チオウリジンの生理作用 : ゲル電気泳動法によるリボ核酸分析

その他

Analysis by the use of polyacrylamide gel electrophoresis was worked out for the comparative studies on chloroplast ribonucleic acids from radish cotyledons grown in the presence and absence of 4-thiouridine, which had been shown to prevent the formation of the functional proteins proper to the chloroplast such as protochlorophyllideholochrome and ribulose 1, 5-diphosphate carboxylase. The rate of de novo biosynthesis of chloroplast ribonucleic acids on illumination in the cotyledons cultured with 4-thiouridine was significantly delayed as compared with that of chloroplast ribonucleic acids from the cotyledons without 4-thiouridine.
On the other hand there was no difference in the formation of ribosomal ribonucleic acids between the 4-thiouridine treated plants and the untreated. The results obtained indicate that 4-thiouridine must have inhibited the development of proplastid (precursor of chloroplast) during the germination stage followed by the growth in the dark.