| File | |
| Title |
ダイコン子葉の葉緑体発生に関する研究(4) : リンゴ酸脱水素酵素について
|
| Title |
Studies on the Chloroplast Development in Radish Cotyledons(4) : on Malate Dehydrogenases
|
| Title Transcription |
ダイコン シヨウ ノ ヨウリョクタイ ハッセイ ニ カンスル ケンキュウ 4 リンゴサン ダツ スイソ コウソ ニツイテ
|
| Creator |
Ochiai Hideo
Kono Yasuhisa
|
| Source Title |
島根大学農学部研究報告
Bulletin of the Faculty of Agriculture, Shimane University
|
| Volume | 7 |
| Start Page | 129 |
| End Page | 134 |
| Journal Identifire |
ISSN 0370940X
|
| Descriptions |
ocalization, properties and time courses of malate dehydrogenases in greening radish cotyledons were studied as well as an inhibitory effect of 4-thiouridine upon the enzymes. Results obtained are as follow.
1) Chloroplast fraction isolated from the radish cotyledon cells contained <NAD>^+ specific malate dehydrogenase, the specific activity of which decreased as the development of the chloroplast proceeded on illumination. 2) Non-chloroplast fraction contained <NADP>^+-malate dehydrogenase and malic enzyme. Cofactor of the <NADP>^+_malate dehydrogenase could be replaced by <NAD>^+ with a slight decrease of the specific activity. Malic enzyme required <NADP>^+ as a specific cofactor. Specific activities of both the enzymes remained unchanged with illumination time. 3) <NAD>^+_malate dehydrogenase of the chloroplast was inhibited by the presence of 4-thiouridine (1-5mM), but malic enzyme in the cytoplasm was not. Based upon these observations, biological significance of <NAD>^+_malate dehydrogenase in the developing chloroplast was discussed. |
| Language |
jpn
|
| Resource Type | departmental bulletin paper |
| Publisher |
島根大学農学部
Shimane University, Faculty of Agriculture
|
| Date of Issued | 1973-12-15 |
| Access Rights | open access |
| Relation |
[NCID] AN00108015
|
