| File | |
| Title |
糸状性,好温性ラン藻Phormidium lapideumの制限酵素
|
| Title |
Restriction Endonucleases from Phormidium lapideum, a Strain of Filamentous and Thermophilic Cyanobacteria
|
| Title Transcription |
シジョウセイ コウ オンセイ ランソウ PHORMIDIUM LAPIDEUM ノ セイゲン コウソ
|
| Creator |
Ochiai Hideo
Ashida Naomi
|
| Source Title |
島根大学農学部研究報告
Bulletin of the Faculty of Agriculture, Shimane University
|
| Volume | 23 |
| Start Page | 184 |
| End Page | 191 |
| Journal Identifire |
ISSN 0370940X
|
| Descriptions |
A couple of restriction endonucleases, Pla I and Pla II, have been purified from a filametous and thermophilic cyanobacterium, Phormidium lapideum. Pla I was proved to be an isoschizomer of Hae III to cleavage the site GG↓CC and was a monomeric protein that had a molecular weight of about 40 kilodaltons. Pla II was an isoschizomer of Nsp (7524) V to recognize the site TTCGAA and was estimated as a heterotetrameric protein (α_2β_2). Pla II has an apparent molecular mass of 176 kilodaltons and that of α subunit was 63 kilodaltons, β subunit 31 kilodaltons. Characteristics of Pla I and Pla II were investigated in comparison with that of the respective isoschizomers, Hae III and Nsp (7524) V.
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
島根大学農学部
Shimane University, Faculty of Agriculture
|
| Date of Issued | 1989-12-21 |
| Access Rights | open access |
| Relation |
[NCID] AN00108015
|
