固定化チアミナーゼIを利用したチアミンおよびそのリン酸エステル類の自動定量法

島根大学農学部研究報告 Volume 18 Page 193-196 published_at 1984-12-20
アクセス数 : 1208
ダウンロード数 : 164

今月のアクセス数 : 79
今月のダウンロード数 : 6
File
d0030018n027.pdf 494 KB エンバーゴ : 2001-10-08
Title
固定化チアミナーゼIを利用したチアミンおよびそのリン酸エステル類の自動定量法
Title
Automatic Determination of Thiamine and Its Phosphates by the Use of Immobilized Thiaminase I
Title Transcription
コテイカ チアミナーゼ 1 オ リヨウシタ チアミン オヨビ ソノ リンサン エステル ルイ ノ ジドウ テイリョウホウ
Creator
Nakamura Toshiie
Fujita Keiji
Source Title
島根大学農学部研究報告
Bulletin of the Faculty of Agriculture, Shimane University
Volume 18
Start Page 193
End Page 196
Journal Identifire
ISSN 0370940X
Descriptions
An automatic method for the determination of thiamine andits phosphates by the application of immobilized thiaminase I was examined.
Thiaminase I catalyzed the base-exchange reaction of the thiamine phosphates as well as thiamine with pyridine, forming heteropyrithiamine(HPT). The contents of thiamine and its phsphates were determined by automatically analyzing HPT formed by the enzyme reaction. It was considered that this system was suitable for the determination of total thiamine.
Language
jpn
Resource Type departmental bulletin paper
Publisher
島根大学農学部
Shimane University, Faculty of Agriculture
Date of Issued 1984-12-20
Access Rights open access
Relation
[NCID] AN00108015