File | |
Title |
固定化チアミナーゼIを利用したチアミンおよびそのリン酸エステル類の自動定量法
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Title |
Automatic Determination of Thiamine and Its Phosphates by the Use of Immobilized Thiaminase I
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Title Transcription |
コテイカ チアミナーゼ 1 オ リヨウシタ チアミン オヨビ ソノ リンサン エステル ルイ ノ ジドウ テイリョウホウ
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Creator |
Nakamura Toshiie
Fujita Keiji
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Source Title |
島根大学農学部研究報告
Bulletin of the Faculty of Agriculture, Shimane University
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Volume | 18 |
Start Page | 193 |
End Page | 196 |
Journal Identifire |
ISSN 0370940X
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Descriptions |
An automatic method for the determination of thiamine andits phosphates by the application of immobilized thiaminase I was examined.
Thiaminase I catalyzed the base-exchange reaction of the thiamine phosphates as well as thiamine with pyridine, forming heteropyrithiamine(HPT). The contents of thiamine and its phsphates were determined by automatically analyzing HPT formed by the enzyme reaction. It was considered that this system was suitable for the determination of total thiamine. |
Language |
jpn
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Resource Type | departmental bulletin paper |
Publisher |
島根大学農学部
Shimane University, Faculty of Agriculture
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Date of Issued | 1984-12-20 |
Access Rights | open access |
Relation |
[NCID] AN00108015
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