| ファイル | |
| 言語 |
英語
|
| 著者 |
Yoshimatsu, K
Tsukuba Division, Research Center for Medicinal Plant Resources, National Institution of Biomedical Innovation, Tsukuba 305-0843, Japan.
Kawahara, N
Tsukuba Division, Research Center for Medicinal Plant Resources, National Institution of Biomedical Innovation, Tsukuba 305-0843, Japan.
Yamamoto, S.-I.
National Institute of Agrobiological Sciences (NIAS), Tsukuba 305-8602, Japan.
Niino, T.
Gene Research Center, University of Tsukuba, Tsukuba 305-8572, Japan.
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| 内容記述（抄録等） | A clonal propagation method by node culture of perilla (Perilla frutescens L. Britton) was investigated. Nodes were plated on solidified 1/2 MS medium with BA and optimum shoot elongation and propagation was obtained at BA 0.05 and 0.1 mg l-1. Cryopreservation using an aluminum cryo-plate was successfully applied to in vitro-grown perilla shoot tips. Excised shoot tips from nodes were precultured on 1/2 MS medium with 0.3 M sucrose and embedded on an aluminum cryo-plate with alginate gel. The cryo-plate with shoot tips was osmo-protected with LS solution and dehydrated in PVS2 for 20 min at 25°C prior to immersion into liquid nitrogen. The recovery growth after cryopreservation was found to be about 80%. This new V-Cryo-plate method has many advantages and may facilitate the cryo-storage of other medicinal plants.
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| 主題 | Cryopreservation
Aluminum
Regrowth
Vitrification
Plants
Clonal propagation
Hypocotyls
Chromosomes
Plant genetics
Japanese culture
|
| 掲載誌名 |
Advances in Horticultural Science
|
| 巻 | 28
|
| 号 | 2
|
| 開始ページ | 79
|
| 終了ページ | 83
|
| 発行日 | 2014
|
| DOI | |
| 出版者 | Dipartimento Di Scienze Delle Produzioni Vegetali, Del Suolo E Dell'Ambiente Agroforestale – DiPSA – University of Florence
|
| 資料タイプ |
学術雑誌論文
|
| ファイル形式 |
PDF
|
| 関連情報 | |
| 著者版/出版社版 |
出版社版
|
| 業績ID | e26030
|
| 部局 |
生物資源科学部
|
