| File | |
| Title |
Immunochemical Studies on Ribonuclease F1
|
| Creator |
Yoshida Hiroshi
Nishioka Masaaki
|
| Source Title |
島根大学医学部紀要
|
| Volume | 33 |
| Start Page | 49 |
| End Page | 52 |
| Journal Identifire |
ISSN 1880084X
|
| Descriptions |
Antibody against ribonuclease (RNase) F1 was raised in a rabbit by intramuscular injection of the protein with Freund’s complete adjuvant. Excess amount of the antibody totally inhibited the enzyme activity on RNA substrate. Quantitative precipitin analysis showed the presence of at least 4 or 5 epitopes (antigenic determinants) on the RNase F1 molecule. Cross-reactivity of the antibody with RNases T1, Ms, U2, St and T2 was examined. Although the double diffusion test detected no cross-reactivity with any of these RNases, Western blot analysis with anti RNase F1 antibody revealed weak cross-reactivity with RNases Ms and T1.
|
| Subjects | |
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
島根大学医学部
Shimane University Faculty of Medicine
|
| Date of Issued | 2010-12-01 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Relation |
[NCID] AA12049432
|
