Detection of cathepsin B-like enzyme activity in mouse epidermis

Shimane journal of medical science Volume 17 Issue 1 Page 5-7 published_at 1999-06-01
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Title
Detection of cathepsin B-like enzyme activity in mouse epidermis
Creator
Ohno Tomoya
Hayashi Shinobu
Chinuki Yuko
Dekio Satoshi
Source Title
Shimane journal of medical science
Volume 17
Issue 1
Start Page 5
End Page 7
Journal Identifire
ISSN 03865959
EISSN 24332410
Descriptions
In this study, we could detect cathepsin B-like, i.e., BANA-hydrolysing, enzymeactivity in the cytosolic fraction of mouse epidermal lysate. The optimum pH and the optimum temperature of this enzyme activity were pH 6.0 and 37℃, respectively. The BANA hydrolysis occured dose dependently to the protein amount of the cytosolic
fraction and, lupeptin, which is a specific inhibitor to cathepsin B, was strongly inhibited BANA hydrolysis at pH 6.0 under 37℃ condition.
Language
eng
Resource Type departmental bulletin paper
Publisher
Shimane Medical University
Date of Issued 1999-06-01
Publish Type Version of Record
Access Rights open access
Relation
[NCID] AA00841586
Remark http://ci.nii.ac.jp/vol_issue/nels/AA00841586_jp.html