File | |
Title |
Detection of cathepsin B-like enzyme activity in mouse epidermis
|
Creator |
Ohno Tomoya
Hayashi Shinobu
Chinuki Yuko
Dekio Satoshi
|
Source Title |
Shimane journal of medical science
|
Volume | 17 |
Issue | 1 |
Start Page | 5 |
End Page | 7 |
Journal Identifire |
ISSN 03865959
EISSN 24332410
|
Descriptions |
In this study, we could detect cathepsin B-like, i.e., BANA-hydrolysing, enzymeactivity in the cytosolic fraction of mouse epidermal lysate. The optimum pH and the optimum temperature of this enzyme activity were pH 6.0 and 37℃, respectively. The BANA hydrolysis occured dose dependently to the protein amount of the cytosolic
fraction and, lupeptin, which is a specific inhibitor to cathepsin B, was strongly inhibited BANA hydrolysis at pH 6.0 under 37℃ condition. |
Language |
eng
|
Resource Type | departmental bulletin paper |
Publisher |
Shimane Medical University
|
Date of Issued | 1999-06-01 |
Publish Type | Version of Record |
Access Rights | open access |
Relation |
[NCID] AA00841586
|
Remark | http://ci.nii.ac.jp/vol_issue/nels/AA00841586_jp.html |