| File | |
| Title |
両生類および哺乳類培養細胞の分裂時における仁の検出
|
| Title |
Cyiochemical Studies for Demonstration oi Nucleoli in Mitotic Divisions of Amphibian and Mammalian Cells in Vitro
|
| Title Transcription |
リョウセイルイ オヨビ ホニュウルイ バイヨウ サイボウ ノ ブンレツ ジ ニ オケル ジン ノ ケンシュツ
|
| Creator |
Seto Takeshi
|
| Source Title |
島根大学教育学部紀要. 自然科学
Memoirs of the Faculty of Education, Shimane University. Natural science
|
| Volume | 8 |
| Start Page | 29 |
| End Page | 36 |
| Journal Identifire |
ISSN 05869943
|
| Descriptions |
Cytochemical methods proposed for the demonstration of nucleoli were comparatively studied in order to obtain more specific visualization of nucleoli with a light microscope.
The amphibian cell culture derived from frog kidney tissue was successfully used in stain preparations of mitotic cells, and some modifications of staining methods resulted effective manifestation of nucleoli at mitotic phases. Moderate extraction of RNA from fixed cells with perchloric acid or RNase was recognized more effective to the subsequent nucleolar stainings for the detection of persistent nucleoli in any kind of cultured cells. |
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
島根大学教育学部
The Faculty of Education Shimane University
|
| Date of Issued | 1974-12-25 |
| Access Rights | open access |
| Relation |
[NCID] AN00107941
|
