ファイル | |
言語 |
英語
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属性 |
Original article
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著者 |
JAHAN, Israt
新林 友美
中島 淳
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内容記述(抄録等) | Non-alcoholic fatty liver disease (NAFLD) is one major chronic liver disease emerging the primarily excessive fat accumulation in the liver. Recent studies have demonstrated that hepatic miRNAs play an important role of NAFLD pathogenesis. Previously we identified up-regulation of miR-126-3p and miR-199a-3p in the patient with NAFLD, but their roles remain unclear. In this study, we investigated the role of miR-126-3p and miR-199a-3p on lipid accumulation in 3T3-L1 adipocytes. Transfection of miR-126-3p and miR-199a-3p enhanced lipid accumulation in 3T3-L1 cells after adipocyte differentiation. Next, we evaluated lipid metabolism-related gene expression by quantitative RT-PCR. MiR-126-3p increased CCAAT/enhancer-binding protein-α (C/EBPα) mRNA expression whereas miR-199a-3p did not affect. Moreover, both miRNAs reduced the expression of hypoxia-inducible factor-1α (HIF-1α) mRNA, predicted target of miRNAs. These results suggested that miR-126-3p and miR-199a-3p promote lipid accumulation in 3T3-L1 adipocytes through regulating HIF-1α and/or C/EBPα mRNA expression.
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主題 | Non-alcoholic fatty liver disease
microRNA- 126-3p
microRNA-199a-3p
lipid deposition
CCAAT/enhancer binding protein-α
hypoxia-inducible factor-1α
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掲載誌名 |
Shimane Journal of Medical Science
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巻 | 39
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号 | 4
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開始ページ | 123
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終了ページ | 133
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ISSN | 03865959
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ISSN(Online) | 24332410
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発行日 | 2022-12
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NCID | AA00841586
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DOI | |
出版者 | Faculty of Medicine, Shimane University
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出版者別表記 | 島根大学医学部
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資料タイプ |
紀要論文
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ファイル形式 |
PDF
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権利関係 | Faculty of Medicine, Shimane University
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権利関係(リンク) | This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
著者版/出版社版 |
出版社版
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部局 |
医学部
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他の一覧 |