ダウンロード数 : ?
言語
日本語
著者
中川 慎介 長崎大・院医歯薬・医科薬理
有賀 純 長崎大・院医歯薬・医科薬理
丹羽 正美 ファーマコセル株式
山形 一雄 日本大・生物資源・食品生命
内容記述(抄録等)
Stroke-prone spontaneously hypertensive rat (SHRSP) is widely accepted as an animal model of hypertension and cerebrovascular disease. SHRSP occurs BBB dysfunction even if chronic hypertension and onset of stroke are not well-established. The properties of BBB are maintained by cross-talk between brain endothelial cells and surrounded adjacent cells, such as astrocytes and pericytes. To reveal the detailed mechanism underlay the BBB dysfunction of SHRSP, we constructed an in vitro BBB model using brain endothelial cells, pericytes, and astrocytes. Isolated brain capillary endothelial cells (BECs) from SHRSP showed leaky barrier function and altered composition of tight junction proteins (claudin-5 and occludin). The co-culture method of BECs and astrocytes indicated that SHRSP astrocytes had less ability to induce barrier function on BECs than did astrocytes derived from normotensive control rat (WKY/Izm). In comparison using the triple co-culture model, SHRSP model showed a weak barrier function than WKY model. These results suggest that BBB-relate cells in SHRSP have different properties and the defective interactions among these cells. This altered cross-talk may be related to occurrences of cerebrovascular diseases in SHRSP.
主題
blood-brain barrier
stroke-prone SHR (SHR-SP)
astrocyte
掲載誌名
日本薬理学会年会要旨集
第93回日本薬理学会年会
ISSN(Online)
2435-4953
出版者
公益社団法人 日本薬理学会
出版者ヨミ
コウエキ シャダン ホウジン ニホン ヤクリ ガッカイ
出版者別表記
Japanese Pharmacological Society
資料タイプ
その他
関連情報
部局
医学部