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ファイル
言語
英語
著者
宗宮 基
加藤 譲
内容記述(抄録等)
We set up a highly sensitive enzyme immunoassay of human growth hormone (hGH) using anti-hGH rabbit Fab'-peroxidase conjugate. IgG was obtained from anti-hGH rabbit serum with salting-out and diethylaminoethyl cellulose. IgG was digested by porcine gastric mucosa pepsin to F (ab')_2 which was reduced to Fab'. Fab' was conjugated with peroxidase by maleimide method. The minimum detectable amount of hGH was 0.3pg/ml using 100μl of dialyzed urine sample without any concentrating procedure. Urinary GH was detectable in all normal subjects. In order to evaluate the measurement of urinary GH as a potential screening test of pituitary disorders, we examined 706 subjects in a population (19 to 80 years old). Urine and plasma samples were collected in the early morning after overnight fasting. Urinary creatinine and β_2-microglobulin levels were measured by autoanalyzer and specific radioimmunoassay, respectively. Urinary GH levels ranged from not detectable to 314 ng/g creatinine in these subjects. The logarithmus of these data showed a normal distribution. The normal range obtained from 58 male and 98 female subjects were 0.5 to 17.0ng/g creatinine and 1.2 to 41.9ng/g creatinine, respectively. Urinary GH levels were elevated in 48 out of the 706 subjects examined (6.8%). However, 13 out of these 48 subjects (1.8%) were acoompanied by increased urinary β_2-microglobulin (>200μg/g creatinine), suggesting that urinary GH levels are influenced by renal tubular dysfunction. In contrast, urinary GH was not detectable in 6 out of the 706 cases (0.8%), suggesting possible hypopituitarism. These results indicate that measurement of urinary GH by highly sensitive enzyme immunoassay was useful for a potential screening test for pituitary dysfunction although the renal function should be considered simultaneously.
主題
sensitive enzyme immunoassay
urinary GH
screening test
掲載誌名
Shimane journal of medical science
13
開始ページ
60
終了ページ
74
ISSN
03865959
ISSN(Online)
24332410
発行日
1992
NCID
AA00841586
出版者別表記
Shimane Medical University
資料タイプ
紀要論文
ファイル形式
PDF
著者版/出版社版
出版社版
部局
医学部
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