Crown Gall形成初期における2,3の知見

French bean stem tissues inoculated with Agrobacterium tumefaciens by needle stabbing were examined under electron-microscope. Twenty-four hours after inocu lation, almost all agrobacteria were in needle-wound site. Undamaged french bean cells face to wound site had a large nucleus. Fourty-eight hours after inoculation, many agrobacteria adhered to french bean cell wall exposed by needle stabbing. Numerous agrobacteria were also observed in french bean cells injured by needle stabbing and intercellular spaces. Seventy-two hours after inoculation, there were many agrobacteria in intercellular spaces apart 2 or 3 cells from wound site. French bean cells in close proximity to agrobacteria showed a vesiculation.