大腸菌群菌株の粘質物(1)

1) Five strains which prodnce a slime substance were selected from about 420 strains of coliforms isolated from fresh water. One of the strains(type : Enterobacter aerogenes 1) showing the most remarkable producibility of the slime substance was studied on the conditions of the production and on the chemical compositions of the slime substance.
2) Among the media studied, sugars-or mannitol-enriched media resulted a good production of the slime substance. Optimum temperature for the production of the slime substance was 22℃ which was far low from the optimum for the growth of this bacteria. Under the anaerobic condition, a considerable growth of the cells was observed, but the slime substance was not produced. An aerobic condition(presence of molecular oxygen) was essential for the production of the slime substance.
3) Thinlayer chromatograms of the acid-hydrolyzates of the slime substance showed that the substance may be a polysacharide consisting of galactose and glucose.