ダウンロード数 : ?
タイトルヨミ
ダイチョウキン ユライ Lガタキン RK4 ノ セイイク ニオケル ナトリウム プロトン コウゴ ギャク ユソウケイ ノ ヤクワリ ニツイテ
タイトル別表記
A Role of Na^+/H^+ Antiporter on Growth of Esherichia coli : L-form RK4
ファイル
言語
英語
著者
新庄 尚史
小野田 哲夫
内容記述(抄録等)
Escherichia coli, L-form NC7 was able to grow over pH range 6.2-8.8 in a complex medium supplemented with 0.34 M-NaCl (NaPY medium) or 0.34 M-KCl (KPY medium). On the other hand, variant strain, L-form RK4 derived from L-form NC7 was able to grow at acidic pH range in KPY medium, but not at all under alkaline pH conditions in both the media. When the NC7 was treated with amiloride or harmaline, inhibitor of Na+ /H+ antiporter, the similar growth inhibition as those of the RK4 was observed in both the media. The Na+ /H+ antiporter activity on everted membrane vesicles from the RK4 was markedly lower than that from the NC7. However, when the RK4 was incubated in alkaline buffer (pH 8.15) significant alkalization of the intracellular pH was not observed. When melibiose. one of substrate for Na-coupled transport system, was added into KPY medium. Na+ was actively transported with melibiose and accumulated in the RK4 cells. In addition, cell swelling and lysis of the RK4 occurred when the cells were incubated in 0.34 M NaCl solution. Therefore, these findings strongly suggest that inability of growth of the RK4 in NaPY medium due mainly to insufficient activity of the Na+ /H+ antiporter in response to extrusion of the exess Na+ accumulated into the RK4 cells.
掲載誌名
島根大学理学部紀要
26
開始ページ
85
終了ページ
94
ISSN
03879925
発行日
1992-12-25
NCID
AN00108106
出版者
島根大学理学部
出版者別表記
The Faculty of Science, Shimane University
資料タイプ
紀要論文
部局
総合理工学部
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