ファイル | |
言語 |
英語
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著者 |
Tanae, Katsuhiro
Department of Appl ied Bioscience and Biotechnology, Facul ty of Li fe and Environmental Science, Shimane Universi ty, Matsue, 690-8504, Japan
Horiuchi, Tomitaka
Department of Appl ied Bioscience and Biotechnology, Facul ty of Li fe and Environmental Science, Shimane Universi ty, Matsue, 690-8504, Japan
Yamakawa, Takuya
Matsuo, Yuzy
Department of Appl ied Bioscience and Biotechnology, Facul ty of Li fe and Environmental Science, Shimane Universi ty, Matsue, 690-8504, Japan
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内容記述(抄録等) | An H3/H4 histone chaperone, Asf1, plays an essent ial role in maintaining genomic stabil i ty in many species, including f ission yeast . Here, we showed that overexpression of a CENP-A chaperone Sim3 suppres sed the temperature sensi t i ve phenotype of asf1-33 and asf1-30 mutants and the defect in chromat in st ructure, and prevented the accumulat ion of DNA damage in asf1-33 mutants at high temperatures. Fur thermore, asf1-33 and Δsim3 were synthet ic lethal . Consistent with this, shutdown of s im3 expression in asf1-33 Δsim3 double mutants that contained extragenic s im3 resul ted in growth retardat ion. In addi t ion, the Δs im3 mutant displayed sens i t ivi ty to thiabendazol and hydroxyurea, which suggests that Sim3 plays a general role in maintaining chromatin st ructur e . Our resul ts sugges t a possibil i ty that Sim3 funct ions as a histone chaperone.
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主題 | Fission yeast
Histone chaperone
Cent romere
Asf1
CENP-A
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掲載誌名 |
FEBS Letters
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巻 | 586
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号 | 23
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開始ページ | 4190
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終了ページ | 4196
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ISSN(Online) | 1873-3468
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発行日 | 2012-11-30
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DOI | |
出版者 | the Federation of European Biochemical Societies Edited by: Felix Wieland
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資料タイプ |
学術雑誌論文
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ファイル形式 |
PDF
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著者版/出版社版 |
著者版
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業績ID | e15542
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部局 |
生物資源科学部
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備考 | This is the peer reviewed version of the following article: https://febs.onlinelibrary.wiley.com/doi/full/10.1016/j.febslet.2012.10.020, which has been published in final form at https://doi.org/10.1016/j.febslet.2012.10.020. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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