The effectiveness of various porous ion-exchange resins in H^+ and OH^+forms on the disinfection of Escherichia coli (Ecoli K-12）has been probed. Water suspensions of up to 10^8 E.coli cells/<cm>___3 were passed through a glass column packed with various ion-exchange resins（0.5～2.0g). The killing effectiveness of the column was determined by using plate counts. Quanternary ammonium strong based anion-exchange resin in OH^-form（IRA-938）, which has enough macropores to encapsulate E.coli cells, was effective to the devitalization of E. coli. The mixed bed of OH^- resins and a sulfonic acid H^+ form cationexchange resin（TAS3-3）, which was noneffective by itself, was found to have remarkable germicidal efficiency by a synergistic effect of OH^- and H^+ ions on the resins. The mixed bed of the two resins has the feasibility of anew and handy "disinfectant" for water treatment.