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ファイル
言語
英語
属性
Original article
著者
GONG Ao
JING Li
内容記述(抄録等)
Type I collagen, which consists of two α1 and one α2 chains, is an abundant extracellular matrix ( ECM ) protein. Changes in type I collagen are often associated with various diseases including fibrosis, osteogenesis imperfecta ( OI ), and Ehlers-Danlos syndrome ( EDS ). In the present study, we developed a method for quantification of type I collagen α1 ( COL1A1 ) in a culture medium of LX-2 human hepatic stellate cells by using nano-liquid chromatography tandem mass spectrometry ( nano-LC/MS/MS ). After selecting a specific peptide of COL1A1, unlabeled and stable isotope-labeled peptides were used for quantitative analysis of COL1A1 by nano-LC/MS/MS. The concentration of secreted COL1A1 in the LX-2 cell culture medium was 38.78 ng/mL. The results indicate that this method is useful for quantifying COL1A1 in a cell culture medium.
主題
type I collagen
COL1A1
nano-LC/MS/ MS
stable isotope-labeled peptide
掲載誌名
Shimane Journal of Medical Science
38
1
開始ページ
1
終了ページ
5
ISSN
03865959
ISSN(Online)
24332410
発行日
2021-03
NCID
AA00841586
DOI
DOI(SelfDOI)
出版者
Faculty of Medicine, Shimane University
出版者別表記
島根大学医学部
資料タイプ
紀要論文
ファイル形式
PDF
権利関係
Faculty of Medicine, Shimane University
著者版/出版社版
出版社版
部局
医学部
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