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タイトルヨミ
コウトウ ショクブツ ニオケル シシツ ブンカイ コウソ ノ ソンザイ ト ソノ バレイショ カイケイチュウ ニオケル サイボウ ナイ ブンプ
日本語以外のタイトル
Occurrence of Lipolytic Acyl-hydrolase in Higher Plants and Its Subcellular Distribution in Potate Tubers
ファイル
言語
英語
著者
松田 英幸
平山 修
内容記述(抄録等)
Lipolytic acyl-hydrolase existed in potato tubers, the leaves of potato, bean, and soybean as well as rice grains with a relatively high activity, but spinach leaves, cabbage, sweet potato, and carrot root showd little or no activity under the present enzyme assay conditions.
During the incubation of the homogenate of potato tubers, the membrane lipids were rapidly hydrolyzed by endogenous lipolytic enzymes. When diisopropyl fluorophosphate (1 mM) was added to the homogenate, the degradation of the membrane lipids was retarded. The subcellular fractionation in the presence and absence of the inhibitor showed that the enzyme was mostly present in the supernatant fraction (about 90% of total activity). But, with other evidence it is possible that the enzyme occurs in some fragile organelles such as vacuoles and was mixed with the soluble fraction by the disruption of the organelles.
The molecular weight of the enzyme in the supernatant and mitochondria fractions was estimated to be 132,000, which differed from that of the purified enzyme (70,000) described previously.
掲載誌名
島根大学農学部研究報告
13
開始ページ
105
終了ページ
111
ISSN
0370940X
発行日
1979-12-15
NCID
AN00108015
出版者
島根大学農学部
出版者別表記
Shimane University, Faculty of Agriculture
資料タイプ
紀要論文
部局
生物資源科学部
備考
開学30周年記念号
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