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タイトルヨミ
ワサビ スミイリビョウ ノ コクショク ブッシツ ニ カンスル ケンキュウ ダイ4ポウ バレイショ バイヨウキ ノ ヘンショク ニ カンスル 2 3 ノ チケン ニツイテ
日本語以外のタイトル
Studies on the Blackish Substances found in Eutrema Wasabi afflicted with Black Leg Disease(4) : Some Knowledges concering the Colour Change of the Culture Medium of Potato
ファイル
言語
日本語
著者
曾我 治
内容記述(抄録等)
Phoma Wasabiae was inoculated on the culuture solution of potato which contained
20% of glucose, and incubated in static state at 25℃.
The process of colour change of the culture medium was studied with regard to the
change of composition of medium and separation of polyphenol from the coloured
culture medium.
1) The colour of the culture medium changes gradually to a reddish orange colour from the surface of medium with the growth of Phoma Wasabiae. The colour changes blackish during 3 or 4 weeks. And then, the absorbance of a visible light at 412 or 413 mμ increases gradually.
2) Under an ultraviolet ray of the wavelength 365 mμ the culture medium has a bluewhite fluorescence, and it changes to a deep green colour with colouring of the medium.
3) Polyphenols were extracted with ethyl acetate from the precipitate which separated from the coloured culture medium with lead acetate solution. The result of study by the use of paper chromatography on the extract, the existence of an yellowish orange coloured substrate was proved. Moreover, it was recognized that when excess petroleum ether was added to the concentrated ethyl acetate extract, an orange-yellow substrate was deposited. Upon recrystallization from aqueous methanol it gave red needles. It was found that the crystal had a trihydroxy-1, 4-naphthoquinone structure, therefore the author proposes the name, "wasabiquinone", for the pigment because of a metabolic product of Phoma Wasabiae. The structure of wasabiquinone will be described in forthcoming reports.
掲載誌名
島根大学文理学部紀要. 理学科編
4
開始ページ
126
終了ページ
135
ISSN
03709434
発行日
1971-03-15
NCID
AN0010806X
出版者
島根大学文理学部
出版者別表記
The Faculty of Literature and Science, Shimane University
資料タイプ
紀要論文
部局
総合理工学部
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