ダウンロード数 : ?
ファイル
言語
英語
属性
Original article
著者
JIAHAO Yang
JIANHU Xu
LU Liu
RUIQIN Cui
内容記述(抄録等)
Chronic heart failure ( CHF ) is one of the most common and also one of the highest mortality diseases, but there is no established effective treatment. The primary molecular mechanism of CHF is the apoptosis of myocardial cells through the PI3K-Akt signaling pathway. So, we investigated the possible mechanism of yang-warming and the fluid retention-resolving method in the treatment of CHF. Methods: The rat model of chronic heart failure was reproduced by intraperitoneal injection of adriamycin. Doppler echocardiography was used to determine rats' cardiac function. ELISA kits detected the levels of BNP and cTnI. H&E staining was used to observe the changes of myocardial pathological morphology in rats. The protein contents of p-PI3K, p-Akt were detected by Western Blot. The expression of Bcl-2 and Bax, and Caspase-8, and Caspase-3 were detected by immunohistochemistry. Results: We revealed that p-PI3K and p-Akt protein expression in the CHF group were decreased, the value of Bcl-2 and Bcl-2/Bax decreased, and the value of Bax, Caspase-8 and 3 increased; All of the treatment groups attenuated the levels of BNP, cTnI, Bax, Caspase-8 and Caspase-3. And the expressions of p-PI3K, p-Akt, Bcl-2, Bcl-2/Bax were increased. The yang-warming group ameliorated the CHF state from the amounts of the releases better than other treatment groups. Conclusions: These revealed that the yang-warming and fluid retention-resolving method might play a role in protecting the myocardium by regulating the PI3K-Akt signaling transduction pathway and subsequently inhibiting apoptosis.
主題
yang-warming and fluid retention-resolving
chronic heart failure
PI3K-Akt pathway
掲載誌名
Shimane Journal of Medical Science
38
1
開始ページ
13
終了ページ
22
ISSN
03865959
ISSN(Online)
24332410
発行日
2021-03
NCID
AA00841586
DOI
DOI(SelfDOI)
出版者
Faculty of Medicine, Shimane University
出版者別表記
島根大学医学部
資料タイプ
紀要論文
ファイル形式
PDF
権利関係
Faculty of Medicine, Shimane University
著者版/出版社版
出版社版
部局
医学部
他の一覧