ダウンロード数 : ?
言語
英語
著者
片倉 賢紀
Sugimoto, Naotoshi
橋本 道男
内容記述(抄録等)
We investigated behavioral thermoregulatory function and acquired heat tolerance of β-amyloid (Aβ)-infused rats. Male Wistar rats were anesthetized and implanted in the intraperitoneal cavity with a temperature transmitter. Aβ peptide (4.9–5.5 nmol) was dissolved in a solvent of 35% acetonitrile and 0.1% trifluoroacetic acid (pH 2.0). The solvent was used as the vehicle. An osmotic pump contained 234 ± 13.9 μl of Aβ solution was subcutaneously implanted in the back and was cannulated into the left cerebral ventricle. Moreover, 0.5 µg of AlCl3 was injected into the right cerebral ventricle with a micro syringe pump (Aβ-infused rats). The solvent-infused rats were used as control rats (CN rats). After 2 weeks, rats were placed in a thermal gradient and their intra-abdominal temperature (Tab) and their ambient temperatures (Ta) selected (Ts) were measured for 3 consecutive days. In an additional study, rats were kept at a Ta of 32°C for 4 weeks to attain heat acclimation. Then, rats were subjected to a heat tolerance test, i.e. they were exposed to a Ta of 36°C for 160 min. Although there were clear day-night variations of Ts and Tab in CN rats, patterns were significantly abolished in Aβ-infused rats. Moreover, heat tolerance obtained by heat acclimation was attenuated in Aβ-infused rats. These results suggest that Aβ-infusion in the lateral ventricle modifies behavioral thermoregulation and lowers an ability to acclimate to heat in rats.
主題
β-amyloid
behavioral thermoregulation
heat acclimation
hypothalamus
rats
掲載誌名
Temperature
2
3
開始ページ
418
終了ページ
424
ISSN
2332-8940
ISSN(Online)
2332-8959
発行日
2015
DOI
PubMed ID
出版者
Landes Bioscience
資料タイプ
学術雑誌論文
部局
医学部