ファイル | |
言語 |
英語
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著者 |
吉田 浩
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内容記述(抄録等) | A convenient method has been developed for investigating interaction of an enzyme with a specific ligand on the basis of the difference absorption spectrum. The experimental procedure has been described in detail. Briefly, to a solution of the enzyme, a concentrated solution of the ligand was added successively in small volume aliquots and the increase in absorbance after each addition was measured at two wavelengths which corresponded to a maximum and a crossover point of the difference absorption spectrum. Processing the obtained data gave the difference at the maximum wavelength as a function of the total ligand concentration. The data set thus generated was fitted to the equilibrium equation and the best fit values for the dissociation constant, K_d and the molar difference absorption coefficient at the maximum wavelength, Aemax, were determined. The method was successfully applied to ribonuclease F1-guanosine 2'-phosphate system and gave the following parameters: K_d=2.5 μM and Δε_<290>=3890 M^<-1>cm^<-1>.
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主題 | enzyme-ligand interaction
difference absorption spectrum
ribonuclease F1
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掲載誌名 |
島根医科大学紀要
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巻 | 18
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開始ページ | 93
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終了ページ | 96
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ISSN | 03879097
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発行日 | 1995-12-01
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NCID | AN00107602
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出版者 | 島根医科大学
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資料タイプ |
紀要論文
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ファイル形式 |
PDF
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著者版/出版社版 |
出版社版
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部局 |
医学部
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他の一覧 |