ファイル | |
言語 |
英語
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著者 |
Haque, Md. Ahsanul
Department of Laboratory Medicine
Abdullah Al Mamun
bDepartment of Physiology, Shimane University School of Medicine, 89-1 Enya Cho, Izumo 693-8501, Japan.
橋本 道男
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内容記述(抄録等) | Mesenchymal stem cell transplantation is demonstrated to improve neurological performance in neurodegenerative diseases including Alzheimer’s disease. To understand the underlying mechanism, we infused amyloid β (Aβ) peptide in the lateral ventricle of adult Wister rats using osmotic pump. After 15 days of continuous infusion, a mesenchymal stem cell line (B10) was transplanted in the lateral ventricle. Learning related behavior was evaluated by 2-way shuttle avoidance test. The results showed that Aβ infusion significantly decreased learning related behavior, whereas that was not decreased in B10 transplanted group. Nissl staining results demonstrated that hippocampal pyramidal neurons in CA1 area was decreased in Aβ-infused rats, whereas that was not decreased in B10 transplanted group. Immunohistochemical examination showed that Aβ mainly deposited in the vessels of the brains of Aβ-infused rats, which was decreased by B10 transplantation. Further analysis showed that B10 transplantation increased vessel density, as revealed by immunofluorescence staining. Moreover, the number of astrocyte and microglia was decreased by Aβ infusion, which was returned to the level of sham animals by B10 transplantation. Real time PCR and immunostaining results showed that B10 transplantation significantly increased IL-1β mRNA and protein expression. Thus, our result showed that MSC transplantation effectively decreased Aβ deposition in cerebral vessel and increased angiogenesis, which could be possible cause of improved neurological performance in Aβ-infused rats.
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主題 | Mesenchymal stem cell
Alzheimer’s disease
amyloid β protein
angiogenesis
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掲載誌名 |
Current Alzheimer Research
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巻 | 15
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号 | 14
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開始ページ | 1331
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終了ページ | 1342
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ISSN | 1567-2050
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ISSN(Online) | 1875-5828
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発行日 | 2018
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DOI | |
PubMed ID | |
出版者 | Saif Zone, Sharjah, U.A.E. ; San Francisco, CA : Bentham Science Publishers, c2004-
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資料タイプ |
学術雑誌論文
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ファイル形式 |
PDF
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著者版/出版社版 |
著者版
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業績ID | e36633
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部局 |
医学部
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学位授与年月日 | 2019-03-22
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学位授与番号 | 甲第663号
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学位名 | 博士(医学)
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学位授与機関 | 島根大学
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学位論文研究科コード |
医学系研究科
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学位授与年度 | 2018
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