ダウンロード数 : ?
ファイル
言語
英語
著者
Moriyama, Daisuke Kaneka Corporation, 1-8, Miyamae-cho, Takasago-cho, Takasago, Hyogo, 676-8688, Japan
Yajima, Kazuyoshi Kaneka Corporation, 1-8, Miyamae-cho, Takasago-cho, Takasago, Hyogo, 676-8688, Japan
Yanai, Ryota Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504, Japan
Ikenaka, Yasuhiro Kaneka Corporation, 1-8, Miyamae-cho, Takasago-cho, Takasago, Hyogo, 676-8688, Japan
Hasegawa, Junzo Kaneka Corporation, 1-8, Miyamae-cho, Takasago-cho, Takasago, Hyogo, 676-8688, Japan
Washida, Motohisa Kaneka Corporation, 1-8, Miyamae-cho, Takasago-cho, Takasago, Hyogo, 676-8688, Japan
Nanba, Hirokazu Kaneka Corporation, 1-8, Miyamae-cho, Takasago-cho, Takasago, Hyogo, 676-8688, Japan
内容記述(抄録等)
Coenzyme Q (CoQ) is composed of a benzoquinone moiety and an isoprenoid side chain of varying lengths. The length of the side chain is controlled by polyprenyl diphosphate synthase. In this study, dps1 genes encoding decaprenyl diphosphate synthase were cloned from three fungi: Bulleromyces albus, Saitoella complicata, and Rhodotorula minuta. The predicted Dps1 proteins contained seven conserved domains found in typical polyprenyl diphosphate synthases, and were 528, 440, and 537 amino acids in length in B. albus, S. complicata, and R. minuta, respectively. E. coli expressing the fungal dps1 genes produced CoQ10 in addition to endogenous CoQ8. Two of the three fungal dps1 genes (from S. complicata and R. minuta) were able to replace the function of ispB in an E. coli mutant strain. In vitro enzymatic activities were also detected in recombinant strains. The three dps1 genes were able to complement an S. pombe dps1, dlp1 double mutant. Recombinant S. pombe produced mainly CoQ10, indicating that the introduced genes were independently functional and did not require dlp1. The cloning of dps1 genes from various fungi has the potential to enhance production of CoQ10 in other organisms.
主題
Coenzyme Q
CoQ
Ubiquinone
decaprenyl diphosphate synthase
掲載誌名
Applied genetics and molecular biotechnology
101
4
開始ページ
1559
終了ページ
1571
ISSN
0175-7598
ISSN(Online)
1432-0614
発行日
2016-11-11
DOI
出版者
Springer
資料タイプ
学術雑誌論文
ファイル形式
PDF
権利関係
© Springer-Verlag GmbH Germany, part of Springer Nature
著者版/出版社版
著者版
業績ID
e31383
部局
生物資源科学部
備考
This is a post-peer-review, pre-copyedit version of an article published in Applied genetics and molecular biotechnology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00253-016-7963-0