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ファイル
言語
英語
著者
Shibata, Tomoko
内容記述(抄録等)
For simultaneous quantitative analysis of glycosaminoglycans (GAGs), we developed a method using LC-electrospray ionization-tandem MS (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode. For the first time, we demonstrated the simultaneous quantification of a total of 17 variously sulfated disaccharides of 4 GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 6 heparan sulfate and 2 keratan sulfate) with a sensitivity of less than 0.5 pmol within 18 min using this method. By one-shot analysis of GAGs with our MS/MS method, we showed the differences in the composition of GAG classes and the sulfation patterns between the porcine articular cartilage and yellow ligament. In addition to the internal disaccharides described above, some saccharides derived from the nonreducing terminal were also simultaneously detected. The simultaneous quantification of both internal and nonreducing terminal saccharides could be useful to estimate the chain length of GAGs. This method would help establish comprehensive GAGomic analysis of biological tissues.
主題
glycosaminoglycan (GAG)
high-sulfated disaccharide
electrospray ionization (ESI)
tandem mass spectrometry (MS/MS)
selected reaction monitoring (SRM)
掲載誌名
Analytical biochemistry
467
開始ページ
62
終了ページ
74
ISSN
0003-2697
ISSN(Online)
1096-0309
発行日
2014-12-15
DOI
出版者
Elsevier
資料タイプ
学術雑誌論文
ファイル形式
PDF
著者版/出版社版
著者版
業績ID
e27382
部局
医学部
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