ダウンロード数 : ?
ファイル
言語
英語
著者
Michikawa, Makoto
Kim, Seung U
内容記述(抄録等)
Oligomer formation is considered as a critical process for the neurotoxic effects of Alzheimer’s amyloid β (Aβ) peptide. Previously we have demonstrated that lysophosphatidylcholine (LPC) increases the oligomer formation of Aβ_<1-42>, the major Aβ peptide found Alzheimer’s disease (AD) lesions. In this study, we have investigated whether LPC affects the neurotoxic effects of Aβ_<1-42> in a neuronal cell line (A1) culture. Dimethyl thiazolyl diphenyl tetrazolium (MTT) assay revealed that up to 10 μM concentration, LPC did not affect A1 cell viability. Aβ_<1-42> decreased the cell viability, and such effect was dose dependently enhanced by LPC. However, neither LPC nor Aβ_<1-42>, alone or in combination increased lactate dehydrogenase (LDH) release from A1 cells after 24-h treatment. Terminal deoxynucleotidyl transferase dUTP-biotin nick-end-labeling (TUNEL) assay showed that LPC increased Aβ_<1-42>-induced apoptotic cell number. To determine the underlying mechanisms, the proteins implicated in apoptosis pathways including Bcl-2- and caspase-family were analyzed by Western blotting. The results demonstrated that Aβ_<1-42> decreased Bcl-2 in A1 cells at 24 h, whereas LPC had no effect at any time point. Both LPC and Aβ_<1-42> increased Bax level at 24 h, and their combined stimulation showed a synergistic effect. Similar synergistic effect of LPC and Aβ_<1-42> on caspase9 activation was observed. Dot blot immunoassay and Western blotting showed that LPC augmented Aβ_<1-42> oligomer formation in cell culture medium. Removing LPC-induced early-formed Aβ_<1-42> oligomer from the culture medium by immunoprecipitation decreased active caspase9 level and neurotoxicity, as revealed by Western blotting and MTT assay. Furthermore, dihydroethidium (DHE) assay showed that Aβ_<1-42> increased reactive oxygen species level in A1 cells, such effect was further enhanced by LPC. Thus, our results demonstrated that LPC increased the oligomer formation process of Aβ_<1-42> peptide in culture condition, and consequently increased apoptotic neuronal death. Such process might be important for the pathogenesis of AD, and inhibition of LPC generation could be a therapeutic target for the disease.
主題
lysophosphatidylcholine
amyloid β
Alzheimer's disease
oligomer
neuronal apoptosis
掲載誌名
Neuroscience
292
開始ページ
159
終了ページ
169
ISSN
03064522
発行日
2015-04-30
DOI
DOI公開日
2017-06-01
PubMed ID
NCID
AA0075489X
出版者
Elsevier
資料タイプ
学術雑誌論文
ファイル形式
PDF
権利関係
Copyright © 2015 IBRO. Published by Elsevier Ltd. All rights reserved.
著者版/出版社版
著者版
業績ID
e28999
部局
医学部
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