ダウンロード数 : ?
ファイル
言語
英語
著者
Sohmiya, Motoi
川口 美喜子
Kato, Yuzuru
内容記述(抄録等)
We set up a rapid and highly sensitive sandwich enzyme immumoassay (EIA) of human insulin using anti-porcine insulin guinea pig Fab'-peroxidase conjugate, and polystyrene balls or polystyrene plates coated with anti-porcine insulin 1gG. The minimal detectable quantity was 0.15μU/ml and O.63μU/ml using 20μ1 sample in polystyrene ball method and polystyTene plate methed, respectively All procedures were completed within three hours. Gel chromatographic analysis of recombinant human insulin showed a single peak of imunoreactive insulin, whereas two peaks, corresponding to insulin and proinsulin, were obtained from plasma obtained from a patient with myotonic dystrophy. The diluted human plasma sample showed linear response parallel lo the standard. Plasma immumoreactive insulin levels measured by EIA were well correlated with those of radioimmunoassay. The EIA systems are accurate enough to measure plasma immunoreactive insulin levels within a few hours without any concentration, suggesting a useful tool for evaluating insulin secretion in clinical and basic research.
掲載誌名
Shimane journal of medical science
17
2
開始ページ
29
終了ページ
35
ISSN
03865959
ISSN(Online)
24332410
発行日
1999-12-01
NCID
AA00841586
出版者別表記
Shimane Medical University
資料タイプ
紀要論文
ファイル形式
PDF
著者版/出版社版
出版社版
部局
医学部
備考
http://ci.nii.ac.jp/vol_issue/nels/AA00841586_jp.html
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