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ファイル
言語
英語
著者
吉田 浩
内容記述(抄録等)
A convenient method has been developed for investigating interaction of an enzyme with a specific ligand on the basis of the difference absorption spectrum. The experimental procedure has been described in detail. Briefly, to a solution of the enzyme, a concentrated solution of the ligand was added successively in small volume aliquots and the increase in absorbance after each addition was measured at two wavelengths which corresponded to a maximum and a crossover point of the difference absorption spectrum. Processing the obtained data gave the difference at the maximum wavelength as a function of the total ligand concentration. The data set thus generated was fitted to the equilibrium equation and the best fit values for the dissociation constant, K_d and the molar difference absorption coefficient at the maximum wavelength, Aemax, were determined. The method was successfully applied to ribonuclease F1-guanosine 2'-phosphate system and gave the following parameters: K_d=2.5 μM and Δε_<290>=3890 M^<-1>cm^<-1>.
主題
enzyme-ligand interaction
difference absorption spectrum
ribonuclease F1
掲載誌名
島根医科大学紀要
18
開始ページ
93
終了ページ
96
ISSN
03879097
発行日
1995-12-01
NCID
AN00107602
出版者
島根医科大学
資料タイプ
紀要論文
ファイル形式
PDF
著者版/出版社版
出版社版
部局
医学部
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