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タイトルヨミ
SEPHAROSE 4B コテイカ チアミナーゼ 1 ノ コウソガクテキ セイシツ
日本語以外のタイトル
Enzymological Proterties of Sepharose 4B-Immobilized Thiaminase I
ファイル
言語
日本語
著者
持田 和男
藤田 啓二
尾添 嘉久
鈴木 喜六
内容記述(抄録等)
The bacterial thiaminases I produced by B. thiaminolyticus strain B and B.thiaminolyticus Matsukawa et Misawa(T.S.B. and T.M.M., respectively) were immobilized by CNBr-activated Sepharose 4B, and their enzymological properties were examined.
The total activities for the immobilized T.S.B. and T.M.M. obtained were 165 and 73 percents, respectively, of those for the native preparations. Although optimal pH and optimal temperature were virtually inchanged by the immobilization, pH- and thermostabilities were considerably increased.
The kinetic parameters(K_m and V_<max>), determined for the base-exchange reaction between thiamine and pyridine at pH 5.5 and 50℃, showed that the immobilization resulted in appreciable increases in both V_<max> and K_m values, except for the K_m value of a thiamine-T.M.M. system.
Upon being stored at 4℃, the immobilized T.M.M. was very stable and its activity was virtually unchanged at 315 days after immobilization whereas the immobilized T.S.B. was considerably unstable. The stability of the immobilized T.M.M. against continuous utilization, determined by a batch method, was also superior to that of the immobilized T.S.B.
These results suggest that the immobilized T.M.M. is more excellent than the immobilized T.S.B. as judged from the viewpoint of their use for catalysts.
掲載誌名
島根大学農学部研究報告
16
開始ページ
177
終了ページ
182
ISSN
0370940X
発行日
1982-12-20
NCID
AN00108015
出版者
島根大学農学部
出版者別表記
Shimane University, Faculty of Agriculture
資料タイプ
紀要論文
部局
生物資源科学部
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