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公開日 : 2003-11-07
540 KB
著者
田島 尚子
磯田 淳
本田 雄一
内容記述(抄録等)
In 2000, lesion mimic mutants of rice were found from cvs. Koshihikari and Nipponbare in eight paddy fields of Shimane Prefecture. Necrotic spots were formed on the leaves of these mutants at the late stage of growth. When 80% ethanol extracts from leaves of each mutant with necrotic spots were spotted on the TLC plates, and B. oryzea were sprayed on the plates, growth inhibition zones were observed. When ethanol extracts were developed by TLC, no difference was observed in TLC-profiles of compounds with positive response to a ninhydrin reagent among mutants. Tryptamine found from mutant rice cv. Sekiguchi-asahi was not detected in each extract from mutants. Antifungal activity was observed in neutral fraction of benzene extract and in acidic fraction of EtoAc extract from ethanol extract, respectively, as demonstrated by growth inhibition and spore germination tests. This result suggested that there were at least two antifungal compounds in the leaves. This study indicated that antifungal compound plays an important role as a possible factor in resistance of lesion mimic mutant of rice.
掲載誌情報
島根大学生物資源科学部研究報告 7 , 9 - 14 , 2002-12-20
出版者
島根大学生物資源科学部
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