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ファイル
言語
英語
著者
田中 克典
松田 英幸
内容記述(抄録等)
In Schizosaccharomyces pombe, a major role of chitin is to build up a complete spore. Here, we analyzed the cda1 + gene (SPAC19G12.03), which encodes a protein homologous to chitin deacetylases, to know whether it is required for spore formation in S. pombe. The homothallic Δcda1 strain constructed by homologous recombination was found to form a little amount of abnormal spores that contained one, two, or three asci, similar to (but not as strong as) the phenotype observed in a deletion mutant of chs1 encoding chitin synthase 1. This phenotype is reversed by expression of S. cerevisiae chitin deacetylase CDA1 or CDA2, suggesting that cda1 encodes a chitin deacetylase. To support the role of Cda1 in sporulation, the timing of expression of cda1 + mRNA increased during sporulation process. We also found that the Cda1 protein self‐associated when its binding was tested both by two‐hybrid system and immunoprecipitation. Thus, these data indicated that cda1 + is required for proper spore formation in S. pombe.
掲載誌名
FEBS Letters
579
12
開始ページ
2737
終了ページ
2743
ISSN
1873-3468
発行日
2005-04-19
DOI
出版者
Federation of European Biochemical Societies
資料タイプ
学術雑誌論文
ファイル形式
PDF
著者版/出版社版
著者版
部局
生物資源科学部
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