ダウンロード数 : ?
ファイル
言語
英語
著者
MATSUO, Yuzy Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
KISHIMOTO, Hayafumi Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
HORIUCHI, Tomitaka Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
TANAE, Katsuhiro Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
内容記述(抄録等)
Gap-repair cloning for plasmid construction in budding yeast is very effective and often used. In contrast, the same method is not widely used in fission yeast, because of a shortage of information on it. Here we describe simple and effective gap-repair cloning for plasmid construction using short tracts of flanking homology. By this method, we combined concentrated DNA fragments with short (20 bp) tracts of flanking homology with the marker gene or the pre-existing gene module. In addition, we found that this method can be applied to one-step cloning of multiple DNA fragments to construct a fusion gene.
主題
fission yeast
gap-repair cloning
short tracts of flanking homology
one-step cloning of multiple DNA fragments
掲載誌名
Bioscience, Biotechnology, and Biochemistry
74
3
開始ページ
685
終了ページ
689
ISSN
0916-8451
ISSN(Online)
1347-6947
発行日
2010-03-23
DOI
出版者
Japan Society for Bioscience, Biotechnology, and Agrochemistry
資料タイプ
学術雑誌論文
ファイル形式
PDF
著者版/出版社版
著者版
部局
生物資源科学部
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