脂質分解酵素処理法によって調べたホウレンソウ葉緑体膜における脂質の分布と機能

Spinach chloroplasts and subchloroplasts were treated with potato lipolytic acyl-hydrolase. The lipids were hydrolyzed through two steps. In the first step a major part of lipids (Type I) was rapidly hydrolyzed without affecting the photoactivity. In the second step the remaining lipids(Type II) were decomposed with a decrease of the photoactivity. The main components of Type II lipids in the photosystem I particles were monogalactosyldiglyceride, and those of the photosystem II particles were phosphatidylglycerol. Molecular species were clearly different between Type I and Type II of monogalactosyldiglyceride, but not in those of other lipid classes. It seems likely that Type II lipids are the bound lipids relating to the photoactivity and Type I lipids are the fluid bilayer lipids which have no concern with the activity.