ダウンロード数 : ?
タイトルヨミ
タンシキン ヘノ プラスミド DNA ドウニュウ ト ソノ ハツゲン ニツイテノ ヨビテキ ケンキュウ
日本語以外のタイトル
Preliminary Study on the Trasfer of Plasmid DNA to Basidiomycetes and its Expression
ファイル
言語
英語
著者
古野 毅
Shimada, Kenta
松田 英幸
上原 徹
内容記述(抄録等)
To attempt the gene transfer to the basidiomycetes such as wood-decaying fungi, the experimental system of transferring plasmid DNA and seeing the expression was investigated. Using two species of Tyromyces palustris and Coriolus versicolor, their protoplasts from mycelia cultivated in SMY liquid culture media were prepared by treatments with the cell wall-lysing enzymes and others. As plasmid DNA, pUC4K having a kanamycin-resistant gene was selected because of obstructing the growth of mycelia. The transfer of plasmid DNA was examined by two ways of polyethylene glycol and electro poration.
The transformants of Tyromyces palustris obtained by electroporation showed obviously a faster growth than the non-transformed cells in kanamycincontaining liquid media. As a result of agarose gel electrophoresis for chromosomal DNA and plasmid DNA extracted from these transformants, the bands could be detected at the same position of plasmid pUC4K. This verified the transformation to Tyromyces palustris by transferring pUC4K.
Keywords: transformation; basidiomycetes; Tyromyces palustris; Plasmid pUC4K; electroporation.
掲載誌名
島根大学農学部研究報告
28
開始ページ
83
終了ページ
92
ISSN
0370940X
発行日
1994-12-20
NCID
AN00108015
出版者
島根大学農学部
出版者別表記
Shimane University, Faculty of Agriculture
資料タイプ
紀要論文
部局
生物資源科学部
他の一覧
このエントリーをはてなブックマークに追加