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ファイル
言語
英語
属性
テクニカルノート
著者
Asano Satoshi
内容記述(抄録等)
Mast cells are highly heterogenic cells meaning that different genes are expressed in each different population of mast cells. So far, it has been very difficult to detect that which individual mast cell expresses which genes in situ. In this study, we amplified the mouse mast cell protease (mmcp)-4 mRNAs by in situ RT-PCR method and successfully visualized the signals of amplicons. It was found that the cells expressing mmcp-4 mRNA were connective tissue type mast cells but not mucosal type mast cells, in mouse tongue and intestine sections. This is the first report that the cells expressing the mast cell-specific gene were detected on histological specimens by in situ RT-PCR method. And it is suggested that this method will be a powerful tool for clarifying the relationships between pathological and physiological changes in diseases and many genes expressed in mast cells.
主題
in situ RT-RCR
mast cells
mouse mast cell protease
掲載誌名
Shimane journal of medical science
33
1
開始ページ
31
終了ページ
38
ISSN
03865959
ISSN(Online)
24332410
発行日
2016-09-30
NCID
AA00841586
出版者
Shimane University Faculty of Medicine
資料タイプ
紀要論文
ファイル形式
PDF
著者版/出版社版
出版社版
部局
医学部
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