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タイトルヨミ
キュウシュウ ニブンプ スル イタボガキカ カキルイ ノ DNA カンテイ
タイトル別表記
DNA fi ngerprinting for Ostreidae oysters in Kyushu
ファイル
言語
日本語
著者
飯塚 祐輔
内容記述(抄録等)
Among several genera of the family Ostreidae, Ostrea, Crassostrea and Saccostrea play a central role in the near shore brackish ecosystem worldwide. Although 9 species of oysters belonging to these 3 genera had been found in Kyushu, Japan, oysters are highly variable in form with ecomorphological variations, and molecular methods can usefully complement morphological in determining the status of oyster taxa. In this study, a simple DNA fi ngerprinting analysis of the mitochondrial DNA segment encoding the 16S ribosomal RNA gene was developed for differentiation of 3 genera oysters. Of 2 Ostrea, 4 Crassostrea, and 2 Saccostrea species distributed in Kyushu, Japan, nucleotide sequences of the mitochondrial DNA 16S ribosomal RNA gene were aligned to detect unique sites of restriction enzymes for genus and species identifi cations by means of PCR-RFLP analysis. PCR amplifi cation of an apparent 530 bp fragment of the partial 16S ribosomal RNA gene was successfully performed on all specimens collected. Direct double digestion of the PCR products using Eco130I and HindIII enzymes enabled to discriminate Ostrea, Crassostrea, and Saccostrea genera. In addition, double and single digestions of the PCR products using DdeI and AluI enzymes and AluI enzyme alone enabled to identify 4 Crassostrea species and 3 Saccostrea species, respectively.
主題
Ostreidae oyster
PCR-RFLP
mitochondrial 16S ribosomal RNA gene
掲載誌名
Laguna : 汽水域研究
15
開始ページ
69
終了ページ
76
ISSN
21852995
発行日
2008-12
NCID
AN10439529
出版者
島根大学汽水域研究センター
資料タイプ
紀要論文
ファイル形式
PDF
著者版/出版社版
出版社版
業績ID
e22301
部局
研究・学術情報機構 エスチュアリー研究センター
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