In the previous paper, the writers isolated antifungal substances from the leaves of several plants. In this paper, leaching of the antifungal substances from theleaves of these plants was studied.
The leaching Pattern was classified as shown in Table 1(I-VIII), and six examples were observed in the tested plants. Annual change of the leaching was found to follow six patterns(A F) as shown in Table 3.
The leaves of several plants, Ginkgo biloba and Yucca gloriosa, had fewer microorganisms, and the plants that had many microorganisms on the leaves were Distylium racemosum, Sapium japonicum, and Dendropanax(Tables 6 and 7).
The ratio between total number of microorganisms and fungi, showed a high value on the leaves of Yucca gloriosa, Acer palmatum, and Dendropanax trifidus, and a lower value in Distylium racemosum(Tables 7 and 9).
The relationship between the leaching of antifungal substances and number of microorganisms on the leaves was studied in these plants. The winters found a decrease in the number of microorganisms on the leaves of tested plants when antifungal substances have leached onto the leaves.