ダウンロード数 : ?
ファイル
言語
英語
著者
Yamamoto, Shin-ichi National Institute of Agrobiological Sciences (NIAS), Tsukuba 305-8602, Japan
Fukui, Kuniaki National Institute of Agrobiological Sciences (NIAS), Tsukuba 305-8602, Japan
Rafique, Tariq National Institute of Agrobiological Sciences (NIAS), Tsukuba 305-8602, Japan
Engelmann, Florent Institut de Recherche pour le Développement (IRD), UMR DIADE, 34394 Montpellier cedex 5, France
Niino, Takao Gene Research Center, University of Tsukuba, Tsukuba 305-8572, Japan
内容記述(抄録等)
Cryopreservation has become a very important tool for the long-term storage of plant germplasm. A new cryopreservation protocol based on air dehydration of explants placed on aluminum cryo-plates, termed the D cryo-plate technique, was developed. In this study, the most suitable conditions of cryopreservation for dormant shoot tips of Japanese persimmon (Diospyros kaki Thunb. ‘Saijo’) using the D cryo-plate technique were investigated. Dormant one-year-old shoots of persimmon were collected from the experimental farm of Shimane University in January 2013 and stored at 2°C until use. After surface sterilization, shoot tips of about 1 mm in size were dissected from the dormant buds and precultured overnight at 25°C on solidified 1/2MS medium containing 0.3 M sucrose. Precultured shoot tips were placed on aluminum cryo-plates and embedded in calcium alginate gel. Osmoprotection of shoot tips was performed by immersing the cryo-plates for 30 min at 25°C in an LS solution containing 2 M glycerol + 1.0 M sucrose in 1/2MS solution. For the D cryo-plate technique, encapsulated shoot tips were dehydrated by placing the cryo-plates in the air current of a laminar flow cabinet for 30–90 min. Cooling was performed by placing the cryo-plates in uncapped cryotubes, which were immersed in liquid nitrogen. For rewarming, the cryo-plates were immersed in 1/2MS medium containing 1.0 M sucrose for 20 min at 25°C. In this study, the preculture did not improve the regrowth after cryopreservation; however, we consider that it should be performed in the D cryo-plate procedure for application of other cultivars and utilization in genebanks. A high regrowth rate of cryopreserved shoot tips (84%) was achieved after dehydration for 30 min. This optimized procedure was applied to 10 additional persimmon cultivars, resulting in regrowth rates ranging between 67 and 97%, with an average of 87%. As shoot tips derived from dormant buds proved to be highly tolerant to liquid nitrogen exposure, the D cryoplate technique may facilitate long-term conservation of persimmon germplasm.
主題
dormant shoot tips
genebank
kaki
osmoprotection
掲載誌名
The Horticulture Journal Preview
84
開始ページ
1
終了ページ
5
ISSN
2189-0102
ISSN(Online)
2189-0110
発行日
2015
DOI
出版者
一般社団法人 園芸学会
出版者ヨミ
イッパン シャダン ホウジン エンゲイ ガッカイ
出版者別表記
The Japanese Society for Horticultural Science
資料タイプ
学術雑誌論文
ファイル形式
PDF
権利関係
© 2015 The Japanese Society for Horticultural Science (JSHS), All rights reserved.
著者版/出版社版
出版社版
業績ID
e26031
部局
生物資源科学部
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