| タイトルヨミ | ダイコン シヨウ ノ ヨウリョクタイ ハッセイ ニ カンスル ケンキュウ 4 リンゴサン ダツ スイソ コウソ ニツイテ
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| 日本語以外のタイトル | Studies on the Chloroplast Development in Radish Cotyledons(4) : on Malate Dehydrogenases
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| ファイル | |
| 言語 |
日本語
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| 著者 |
落合 英夫
河野 泰久
柴田 均
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| 内容記述（抄録等） | ocalization, properties and time courses of malate dehydrogenases in greening radish cotyledons were studied as well as an inhibitory effect of 4-thiouridine upon the enzymes. Results obtained are as follow.
1) Chloroplast fraction isolated from the radish cotyledon cells contained <NAD>^+ specific malate dehydrogenase, the specific activity of which decreased as the development of the chloroplast proceeded on illumination. 2) Non-chloroplast fraction contained <NADP>^+-malate dehydrogenase and malic enzyme. Cofactor of the <NADP>^+_malate dehydrogenase could be replaced by <NAD>^+ with a slight decrease of the specific activity. Malic enzyme required <NADP>^+ as a specific cofactor. Specific activities of both the enzymes remained unchanged with illumination time. 3) <NAD>^+_malate dehydrogenase of the chloroplast was inhibited by the presence of 4-thiouridine (1-5mM), but malic enzyme in the cytoplasm was not. Based upon these observations, biological significance of <NAD>^+_malate dehydrogenase in the developing chloroplast was discussed. |
| 掲載誌名 |
島根大学農学部研究報告
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| 巻 | 7
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| 開始ページ | 129
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| 終了ページ | 134
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| ISSN | 0370940X
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| 発行日 | 1973-12-15
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| NCID | AN00108015
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| 出版者 | 島根大学農学部
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| 出版者別表記 | Shimane University, Faculty of Agriculture
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| 資料タイプ |
紀要論文
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| 部局 |
生物資源科学部
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| 他の一覧 |
