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タイトルヨミ
ダイコン シヨウ ノ ヨウリョクタイ ハッセイ ニ カンスル ケンキュウ 4 リンゴサン ダツ スイソ コウソ ニツイテ
日本語以外のタイトル
Studies on the Chloroplast Development in Radish Cotyledons(4) : on Malate Dehydrogenases
ファイル
言語
日本語
著者
落合 英夫
河野 泰久
柴田 均
内容記述(抄録等)
ocalization, properties and time courses of malate dehydrogenases in greening radish cotyledons were studied as well as an inhibitory effect of 4-thiouridine upon the enzymes. Results obtained are as follow.
1) Chloroplast fraction isolated from the radish cotyledon cells contained <NAD>^+ specific malate dehydrogenase, the specific activity of which decreased as the development of the chloroplast proceeded on illumination.
2) Non-chloroplast fraction contained <NADP>^+-malate dehydrogenase and malic enzyme. Cofactor of the <NADP>^+_malate dehydrogenase could be replaced by <NAD>^+ with a slight decrease of the specific activity. Malic enzyme required <NADP>^+ as a specific cofactor. Specific activities of both the enzymes remained unchanged with illumination time.
3) <NAD>^+_malate dehydrogenase of the chloroplast was inhibited by the presence of 4-thiouridine (1-5mM), but malic enzyme in the cytoplasm was not.
Based upon these observations, biological significance of <NAD>^+_malate dehydrogenase in the developing chloroplast was discussed.
掲載誌名
島根大学農学部研究報告
7
開始ページ
129
終了ページ
134
ISSN
0370940X
発行日
1973-12-15
NCID
AN00108015
出版者
島根大学農学部
出版者別表記
Shimane University, Faculty of Agriculture
資料タイプ
紀要論文
部局
生物資源科学部
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